Short term DPA ( 22 : 5 n - 3 ) supplementation increases tissue DPA , DHA and EPA 1 concentration in rats
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چکیده
49 50 The metabolic fate of dietary n-3 docosapentaenoic acid (DPA) in mammals is currently 51 unknown. The aim of this study was to determine the extent of conversion of dietary 52 DPA to docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in rats. Four 53 groups of male weanling Sprague Dawley rats (aged 5 weeks) were given 50 mg of 54 DPA, EPA, DHA or oleic acid, daily for 7 days by gavage. At the end of the treatment 55 period the tissues were analysed for concentrations of long chain polyunsaturated fatty 56 acids (PUFA). DPA supplementation led to significant increases in DPA concentration 57 in all tissues, with largest increase being in adipose (5 fold) and smallest increase being 58 in brain (1.1 fold). DPA supplementation significantly increased the concentration of 59 DHA in liver and the concentration of EPA in liver, heart and skeletal muscle, 60 presumably by the process of retroconversion. EPA supplementation significantly 61 increased the concentration of EPA and DPA in liver, heart and skeletal muscle and the 62 DHA concentration in liver. DHA supplementation elevated the DHA levels in all 63 tissues and EPA levels in the liver. Adipose was the main tissue site for accumulation of 64 DPA, EPA and DHA. This data suggests that dietary DPA can be converted to DHA in 65 the liver, in a short term study, and that in addition it is partly retro-converted to EPA in 66 liver, adipose, heart and skeletal muscle. Future studies should examine the 67 physiological effect of DPA in tissues such as liver and heart. 68
منابع مشابه
Short-term docosapentaenoic acid (22:5 n-3) supplementation increases tissue docosapentaenoic acid, DHA and EPA concentrations in rats.
The metabolic fate of dietary n-3 docosapentaenoic acid (DPA) in mammals is currently unknown. The aim of the present study was to determine the extent of conversion of dietary DPA to DHA and EPA in rats. Four groups of male weanling Sprague-Dawley rats (aged 5 weeks) were given 50 mg of DPA, EPA, DHA or oleic acid, daily for 7 d by gavage. At the end of the treatment period, the tissues were a...
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